Our Free White Papers are ideal for researchers new to 3D cell culture. Download them today to learn more about this paradigm changing technology!
This white paper illustrates the importance of three-dimensional (3D) cell culture in the production of pluripotent stem cell (PSC)-derived models of embryo development and differentiation. It contains a general overview of embryoid bodies (EBs) and a comparison between the various methods used for their generation. There is also a discussion about using teratoma assays versus EBs to validate pluripotency with newly derived PSC lines. Finally, there is a brief description of recent publications illustrating how 3D culture can be used to maximize differentiation. Download the white paper.
Numerous cancer model systems are available to investigate disease mechanisms and to screen therapies. While all of the models have contributed critical information about cancer biology, current tools have significant problems. Roughly 90% of promising preclinical drugs, in all therapeutic classes, fail to result in efficacious human treatments, thereby wasting vast amounts of time and money and, ultimately, delaying the discovery of successful interventions. In the most simplistic view, two-dimensional (2D) tissue culture models lack realistic complexity, while animal models are expensive, time consuming, and too frequently fail to reflect human tumor biology. This white paper presents five alterations in cellular physiology that suggest why three-dimensional (3D) cultures of cancer cells are a scientifically-rigorous method to generate new drug candidates before moving to expensive and time-consuming animal models. Download the white paper.
For decades, three-dimensional (3D) cell culture has been employed by tissue engineers, stem cell scientists, cancer researchers and cell biologists, largely in university settings. The development of new materials or methods has been driven by the desire of these scientists to incorporate experimental systems that better represent the in vivo environment into their research. Early adopters of 3D cell culture technology have reaped the benefits of better data with groundbreaking knowledge of tissue and cancer behavior. Download the white paper.
It is often said that the main expense in drug discovery is failure, and oncology drug discovery is no exception. In 2011, nearly 900 anti-cancer medicines and vaccines were in clinical trials or under the Federal Drug Administration (FDA) review , yet only 12 oncology drugs were approved in that year . With an average clinical trial length of six years, these numbers represent hundreds of drugs in clinical trials that will not make it to market. Behind those hundreds of unsuccessful drug candidates lie hundreds of millions of dollars spent in research and development (Table 1) and clinical trials costs. Download the white paper.
Three-dimensional (3D) cell culture is motivated by the strong and growing need to carry out experiments in cellular models that better mimic physiological tissues. Conventional two-dimensional (2D) cell cultures often fail to capture the cellular functions and responses that are present in tissue. As a result, drug assays and biological research findings based on conventional 2D cell cultures tend to be skewed and offer limited predictive capability. Download the white paper.